Yb Integrates piRNA Intermediates and Processing Factors into Perinuclear Bodies to Enhance piRISC Assembly
نویسندگان
چکیده
منابع مشابه
UAP56 Couples piRNA Clusters to the Perinuclear Transposon Silencing Machinery
piRNAs silence transposons during germline development. In Drosophila, transcripts from heterochromatic clusters are processed into primary piRNAs in the perinuclear nuage. The nuclear DEAD box protein UAP56 has been previously implicated in mRNA splicing and export, whereas the DEAD box protein Vasa has an established role in piRNA production and localizes to nuage with the piRNA binding PIWI ...
متن کاملExport of piRNA precursors by EJC triggers assembly of cytoplasmic Yb-body in Drosophila
PIWI-interacting RNAs (piRNAs) are effectors of transposable element (TE) silencing in the reproductive apparatus. In Drosophila ovarian somatic cells, piRNAs arise from longer single-stranded RNA precursors that are processed in the cytoplasm presumably within the Yb-bodies. piRNA precursors encoded by the flamenco (flam) piRNA cluster accumulate in a single focus away from their sites of tran...
متن کاملRoles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila.
PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs...
متن کاملRecruitment of Armitage and Yb to a transcript triggers its phased processing into primary piRNAs in Drosophila ovaries
Small RNAs called PIWI -interacting RNAs (piRNAs) are essential for transposon control and fertility in animals. Primary processing is the small RNA biogenesis pathway that uses long single-stranded RNA precursors to generate millions of individual piRNAs, but the molecular mechanisms that identify a transcript as a precursor are poorly understood. Here we demonstrate that artificial tethering ...
متن کاملPerinuclear sudanophil bodies in mammalian epidermis.
1. In the epidermis of laboratory mammals there are perinuclear lipid spherules which are demonstrated clearly with sudan black. The centre of the larger of these droplets is occupied by a sudanophobe core of varying dimensions. 2. The distribution of the bodies corresponds to the osmiophil elements in comparable tissues, and the structural pattern is similar to that of the Golgi elements in ot...
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ژورنال
عنوان ژورنال: Cell Reports
سال: 2014
ISSN: 2211-1247
DOI: 10.1016/j.celrep.2014.05.043